碱性过氧化氢酶高产菌的筛选、鉴定及发酵条件优化

Screening, Identification and Fermentation Optimization of a Alkaline Catalase-Producing Strain

摘要:

从纺织厂的车间污水分离获得菌株WSHDZ-01，其产过氧化氢酶酶活为 600 U/mL，酶活的pH范围为5.0～12.0。根据对该过氧化氢酶生产菌的形态和生理生化特征的分析和16S rDNA序列分析，鉴定该菌为枯草芽孢杆菌。通过对该菌株发酵培养基中碳氮源的优化，其最佳碳源为10 g/L的葡萄糖，氮源为5 g/L的 NaNO₃，在此条件下产酶达3258 U/mL。此外，该菌株所产过氧化氢酶的最适反应pH为11.0，最适温度为55℃，在pH11.0和50℃下保持15min后，剩余酶活仍达98％以上。

Abstract:

A strain named as WSHDZ-01 with high capability of producing alkaline catalase（CAT）was isolated from the wastewater discharged from a textile factory. The catalase from this strain was active in the range of pH 5.0-pH12.0, and the highest enzyme activity was observed as 600 U/mL at pH 7.0. According to the physiological and biochemical characteristics and 16S rDNA sequence of WSHDZ-01, the strain was identified as Bacillus subtilis. The optimal carbon source and nitrogen source were also determined as 10 g/L glucose and 5 g/L NaNO₃, respectively. The highest enzyme activity of 3258 U/mL was achieved after the optimization. The resultant catalase reacted best at 55 ℃ and pH 11.0, and had activities > 98% after 15 min maintenance under 50℃ and pH 11.0.