酒色着色菌利用产酸克雷伯氏菌发酵废液产氢

Hydrogen Production by Chromatium vinosum with Fermentation Waste Produced by Klebsiella oxytoca

摘要:

研究了酒色着色菌(Chromatium vinosum DSM185)利用产酸克雷伯氏菌（Klebsiella oxytoca HP1）发酵产氢废液进行光发酵和暗发酵产氢的可行性，以达到对产氢底物的充分利用和对产氢废液的进一步处理。研究结果表明C.vinosum可以利用K.oxytoca的发酵废液进行光发酵产氢和暗发酵产氢。C.vinosum发酵产氢后废液中残余还原糖和主要有机酸（丁酸）的含量明显降低，发酵产氢的最佳pH为6.5，添加0.1%(W/W)NH₄Cl能促进产氢。在光照条件下丁酸利用率可达54.38%，产氢量达36.97 mL/mg；在黑暗条件下丁酸利用率可达36.01%，产氢量达37.50mL/mg。

Abstract:

Photosynthetic bacteria(PSB) showed great promise in biohydrogen production. Chromatium vinosum was able to utilize the fermentation waste of Klebsiella oxytoca for both photo-fermentative and dark-fermentative hydrogen production. The content of residual sugars and main organic acids decreased obviously after hydrogen production by C.vinosum. The maximal hydrogen production of C.vinosum was obtained at pH 6.5 adding extra 0.1%(W/W) NH₄Cl. Under photo-fermentative conditions, the content of butyric acid decreased by 54.38%, and the maximal hydrogen yield was 36.97 mL/mg cell. Under dark-fermentative conditions, the content of butyric acid decreased by 36.1% and the maximal hydrogen production was achieved as 37.50 mL/mg cell.