中性植酸酶高产菌株的筛选及产酶条件研究

Studies on Screening and Conditions of Strains Highly Producing Neutral Phytase

摘要:

以地衣芽孢杆菌为原始出发菌株，用紫外线反复诱变，最终获得一株中性植酸酶高产菌株B.licheniformis LL8，其酶活性约为原始出发菌株的2倍。该菌株具有稳定的遗传性能。通过单因素试验和正交试验对发酵条件进行了优化，当培养温度为55℃，pH为7.5，接种量为10％时，经30h培养后，中性植酸酶活力最高达到2268.4U/mL。

Abstract:

A highly phytase-producing strain B.licheniformis LL8 was obtained by several mutagenesis of UV with B.licheniformis as starting strain.The new strain produced about two folds of phytase activity as compared with the starting strain. The production performance of the strain was stable. The cultivation conditions were optimized by single factor and orthogonal experiment. When the mutant B.licheniformis LL8 was cultivated at 55℃, initial pH 7.5 with the inoculation size of 10% for 30h in WBE medUm, the phytase activity was up to 2268.4U/mL.