均匀设计法优化烟管菌产漆酶培养基

Optimization of Culture Medium for Laccase Production from Bjerkandera adusta by Uniform Design

摘要:

应用均匀设计、二次多项式逐步回归分析对烟管菌(Bjerkandera adusta)WZFF.W-Y11产漆酶液态发酵培养基进行优化。结果表明，培养基组成为麸皮水解液1%、淀粉24.0 g/L、葡萄糖24.0 g/L、豆饼粉4.8 g/L、NH₄Cl 3.2g/L、KH₂PO₄ 3.2 g/L、MgSO₄·7H₂O 0.2 g/L、CuSO₄ ·5H₂O 0.006 g/L，起始pH6.5，在28℃、150r/min、250mL的摇瓶培养条件下可以稳定地获得9672U/L的漆酶活力。

Abstract:

Both uniform design and stepwise regression were employed in this study to optimize culture medium for laccase production from Bjerkandera adusta WZFF.W-Y11. The optimal fermentation parameters of B. adusta WZFF.W-Y11 in shaking flask were as follows: starch 24.0g/L, glucose 24.0g/L, bean cake powder 4.8g/L, NH₄Cl 3.2g/L, KH₂PO₄ 3.2g/L, MgSO₄·7H₂O 0.2 g/L, CuSO₄·5H₂O 0.006 g/L, hydrolysate from wheat bran 1％, initial pH6.5, rotating speed 150 r/min, culture temperature 30℃. The activity of laccase reached 9672 U/L.