变质豆浆中腐败微生物的分离与初步鉴定

Separation and Preliminary Identification of Spoilage Organisms in Transmutative Soy Milk

摘要:

从5种变质豆浆中分离得到3株腐败菌株S₁、S₂和S₃。3株菌均能在1×105Pa、30 min杀菌条件下和添加300mg/kg Nisin杀菌条件下存活。对分离菌株进行了菌落形态、生理生化特征和16S rDNA序列分析, 鉴定结果表明3株菌均为革兰氏阴性细菌，分别为地衣芽孢杆菌(Bacillus licheniformis)、短小芽孢杆菌(Bacillus pumilus)和短芽孢杆菌(Brevibacillus borstelensis)，GenBank登录号为EF439666～EF439668。

Abstract:

In this paper, three spoilage organisms were separated from five transmutative soy milks, and all the three spoilage bacteria could survive condition of both 1×10₅Pa，30min and 300mg/kg Nisin. Morpha character, physiological and biochemical characteristics, and a phylogenetic analysis based on 16S rDNA gene sequences reveal that these three strains are Bacillus licheniformis, Bacillus pumilus and Brevibacillus borstelensis respectively. GenBank accessions for these three strains are EF439666-EF439668。