酯酶产生菌株的分离筛选

The Screening of Microorganism Producing Esterase

摘要:

利用溴甲酚紫染色法和发酵测酶活的方法，从土壤中筛选出一株酯酶高产菌株ZM1。对ZM1进行形态特征及5.8S rDNA基因两侧的内转录间隙进行序列分析推测该菌株为灰绿犁头霉(Absidia glauca Hagem)。经过紫外诱变处理，得到1株正突变株ZMM1，在适合条件下，酶活达到58.76U/mL,比出发菌株提高了104.3％。传代实验表明ZMM1具有良好的遗传稳定性。

关键词:灰绿犁头霉，酯酶，筛选，鉴定，紫外诱变

Abstract:

A strain named ZM1 capable of producing esterase had been isolated from soil samples by the methods of bromocresol purple staining technique and the determination of esterase value. The strain was identified as Absidia glauca Hagem based on its morphological characteristics and the sequence similarity of ribosomal DNA-ITS. A strain named ZZM1 was selected after UV mutagenesis,The results proved that ZMM1 had steady transmissibility,when ZMM1 was cultivated at 32℃ for 48h with 160r/min, the yields of esterase was 58.76U/mL,104.3% higher than ZM1.

Key words:Absidia glauca Hagem,Esterase, Screening, Identification, UV mutagenesis