真菌B-39与虎杖悬浮细胞的共培养

Dual Culture of Fungus and Suspension Polygonum cuspidatum Cell

摘要:

初步探讨了虎杖来源真菌B-39与虎杖悬浮细胞之间的共培养关系及其白藜芦醇的积累特征。通过肉眼和光学显微镜观察真菌B-39与虎杖悬浮细胞的形态结构及生长情况，并用HPLC检测其白藜芦醇的积累特征，结果表明，两者均能在共培养体系中正常生长，且生长量可达4.908g，但白藜芦醇的含量下降了50μg/mL，而真菌接种量的大小可与虎杖悬浮细胞共培养建立一种动态平衡体系，由此推测真菌B-39与虎杖悬浮细胞可能为共生状态。

Abstract:

To study the dual culture relationship of fungus B-39 from Polygonum cuspidatum and suspension Polygonum cuspidatum cell and their accumulated features of resvertrol, the morphology and growth status of fungus B-39 and suspension P.cuspidatum cell and the resvertrol accumulated features were investigated. The results showed that they could normally grow in the dual culture system, and the growth of the dual cultural group was 4.908 g, but the content of resvertrol was reduced to 50μg/mL. A dynamic balanced system was established through controlling the fungal inoculums. It is presumed that fungus B-39 and suspension P.cuspidatum cell could be a balanced antagonism.