碳源和氮源对黑盖木层孔菌菌丝生长的影响

Studies on the Effects of Different Carbon Sources and Nitrogen Sources on the Growth of Phellinus Nigricans

摘要:

研究了四种碳源（蔗糖、乳糖、葡萄糖、可溶性淀粉）和九种氮源（玉米面、麸皮、马铃薯、大豆粉、酵母粉、蛋白胨、硝酸钾、硝酸铵、尿素）对黑盖木层孔菌菌丝生长的影响。从不同代数的菌丝体中提取多糖，并测定多糖含量、分子量分布范围及单糖组成。结果表明：黑盖木层孔菌菌丝生长的最佳碳源为可溶性淀粉，最佳氮源为玉米面，最优碳氮组合为蔗糖和玉米面的组合。不同代数的菌丝体多糖性状基本稳定。

Abstract:

The effects of four kinds of carbon sources and nine kinds of nitrogen sources on the fungus Phellinus nigricans mycelia were studied. The four kinds of carbon source are sucrose, lactose, glucose, soluble starch.The nine kinds of nitrogen source are corn meal, wheat bran, potato, soybean meal, yeast extract powder, peptone, potassium nitrate, ammonium nitrate, and urea. Polysaccharides were extracted from different generations of mycelia and the contents, monosaccharide components and molecular weight were analyzed. The results were that the optical carbon source and nitrogen source were starch and corn meal. The optical combination was sucrose and corn meal. The properties of polysaccharides from different generations of mycelia were stable.