一株对Monacolin K有转化作用菌株的鉴定

Primarily Identification of the Strain Bioconverting Monacolin K

摘要:

从1000多株放线菌中筛选出对Monacolin K有转化作用的菌株并对其进行初步鉴定。结果发现菌株ST2710的气丝交替或不规则分枝，不形成螺旋；可利用多种碳源；牛奶不凝固，不胨化；不利用纤维素，水解淀粉；通过对其细胞化学组分的分析，结果表明菌株ST2710细胞壁为Ⅳ型，糖型为A型，醌组分为MK-9（H₄,H₆），不含枝菌酸，G+C含量为67.3%（摩尔比）；将该菌株的16S rDNA序列与GenBank数据中已有序列进行比对，发现其序列与Amycolatopsis 属菌株的16S rDNA序列相似性最高，达到99%以上。初步将菌株ST2710 归类为Amycolatopsis sp.

Abstract:

In this paper,approximately 1000 strains of actinomycete were screening for their ability for microbial transformation of Monacolin K.Strain ST2710 were capable of bioconverting Monacolin K into hydroxy-Monacolin K.Strain ST2710 was primarily identified using the medium employed by Waksman,Shirling and Gottlieb and the current ISP medium.On Gause's starch agar medium,aerial mycelium form branching filaments and do not show coiling.The utilization of carbohydrates by the ST2710 strain include glucose,sucrose,starch,glycerin etc and determination was made after cultivation at 28℃ for 14 days.It could not decompose milk and liquefy gelatin.It could hydrolysate starch and not grow on cellulose.Strain ST2710 contain major amounts of meso-diaminopimelic acid as the wall diamino acid,arabinose and galactose as major wall sugars;but does not contain mycolic acids.The G＋C content of the DNA is 67.3 mol%.16S rDNA analysis indicated membership of strain ST2710 to the genus Amycolatopsis (99%).Therefore,according to the references,the ST2710 strain was primarily identified as Amycolatopsis sp.