Burkholderia cepacia X4降解油脂特性研究

Biodegradation of Oil and Grease by Burkholderia cepacia X4

摘要:

从长期受油污染的土壤中分离筛选得到的Burkholderia cepacia X4菌株能高效降解油脂。该菌株降解油脂的最适温度和pH分别为30℃和7.0，菌株降解油脂时适宜的氮源为硫酸铵，适宜碳氮比为4∶1。共基质碳源的添加有利于生物量的迅速增加和油脂降解率的提高，添加适量的葡萄糖能使油脂降解率提高8%～10%。50 mg/L Ca²⁺对菌株生长和油脂降解更有利。在橄榄油浓度高达20g/L条件下最大油脂降解率仍可达83%。在油脂浓度≤2500mg/L时，该菌对油脂的降解符合抑制动力学Monod方程。

关键词:洋葱伯克霍尔德氏菌(Burkholderia cepacia)，生物降解，油脂

Abstract:

Strain Burkholderia cepacia X4 was isolated from long time oil polluted soil,which was able to degrade oil and grease effectively.The optimal temperature and pH for oil degradation were 30℃ and 7.0 respectively.The suitable nitrogen source,C/N and Ca²⁺ for strain growth and oil degradation were (NH₄)₂SO₄,4∶1 and 50mg/L respectively.Addition of co-substrate carbon source increased biomass and oil degradation rate.With proper quantities of glucose,the oil degradation rate is increased by 8%～10%.When the concentration of olive oil reach 20g/L,the maximum oil biodegradation rate can still reach 83%.Oil degradation by Burkholderia cepacia X4 followed Monod kinetics model in case of olive oil ≤2500mg/L.

Key words:Burkholderia cepacia, Biodegradation, Oil and grease