酸胁迫对干酪乳杆菌H+-ATP酶基因表达的影响

Expression of H+-ATPase Gene Associated with Different AcidicCondition in Lactobacillus casei

摘要:

耐酸性是乳酸菌重要的益生菌性状之一。实验采用半定量RT-PCR法分别对在不同酸度条件下培养后的3株不同干酪乳杆菌的H⁺-ATP酶基因mRNA表达水平进行了比较和分析。实验结果显示，随着培养基酸度增加干酪乳杆菌的生长受到抑制，特别在pH4.0条件下干酪乳杆菌的生长受到强烈的抑制；H⁺-ATP酶基因的表达量随着培养基酸度的增加而增加。推测H⁺-ATP酶与干酪乳杆菌耐受酸性条件是有一定的关联的。

Abstract:

The ability of acid tolerance is one of the most important properties of lactic acid bacteria.To investigate the gene expression of three stains of Lactobacillus casei which incubated in different acidic condition,a semi-quantitative RT-PCR method was used to detect the H⁺-ATPase gene expression in different acidic condition.The result indicated that the growth of Lactobacillus casei had been restrained by the acid stress，especially when the incubated condition changed to pH4.0,and the expression of H⁺-ATPase gene was increased along with the increased of incubated condition acidity.We guess it has some relationship between H⁺-ATPase and the growth ability of Lactobacillus casei in different acidic condition.