TTC-CaCO3复合平板法快速筛选丙酮酸高产菌株

Screening of High-pyruvate-producing Strain Using by TTC-CaCO3 Complexes Medium

摘要:

设计出一种用于快速筛选丙酮酸高产菌株的方法。这是一种理性化筛选方法。其原理是菌落产生的丙酮酸与碳酸钙反应形成可溶性的丙酮酸钙而出现透明圈，比较透明圈直径可以直观的选择出产酸较多的菌株；TTC与ADH作用而显色，颜色的深浅与ADH活性呈正比，这样可监测丙酮酸向乙醇的转化，筛选出低转化率的菌株。通过此方法，筛选出产酸多、ADH酶活力弱、发酵副产物少的目的菌株。

Abstract:

It designs a way that can easily screen high-pyruvate-producing strain. It is a intelligently selected method which can highly improve the efficiency of strain screening. The principle can be described as the following: On the CaCO₃ medium, a transparent ring can be exhibited based on the reaction of PYR produced by the strain and CaCO₃ in the medium for pyruvate-calcium is a kind of soluble substance, it is obviously that the high-pyruvate-producing strain has a bigger dimension of the transparent ring. On the other hand, color reaction between TTC and ADH indicate the enzyme activities which have a proportional relation with color, our object strain is a weak-ADH-enzyme- activities type with a weak metabolic flux from PYR to alcohol. So the white color strain may be the right choice.