绿色木霉菌LTR-2孢子提取物的抑菌活性及化学成分分析

Analysis of Chemical Components and Antifungal Activity of Extraction from Conidia of Trichoderma viride LTR-2

摘要:

通过研究绿色木霉菌LTR-2分生孢子提取物的抑菌活性及化学成分，为进一步提取纯化新型抗生素提供依据。采用固体麸皮培养基培养绿色木霉菌LTR-2，以二氯甲烷浸提法提取分生孢子中的抗菌物质，采用菌丝生长法测定提取物的抑菌活性，并用气相色谱-质谱联用仪进行分析，峰面积归一法计算有关成分的相对含量。绿色木霉菌LTR-2分生孢子的提取物抑菌谱广，对供试11种植物病原真菌均有不同程度的抑制作用；抑制效果好，对禾谷丝核菌的抑制率为89.3%。从提取物中分离鉴定出60多种化学成分，其中烷烃类成分数量最多，为43种，其他成分有酮类、有机酸类、醇类、烯类等，主要成分是麦角固醇，含量为41.90%。结论：绿色木霉菌LTR-2分生孢子提取物具有抑菌作用。通过化学成分分析，提取物中含有化合物5,6-二氢-6-戊基-2H-吡喃-2-酮，含量为2.35%，结合文献报道，推测5,6-二氢-6-戊基-2H-吡喃-2-酮是提取物中起抑菌作用的物质。

Abstract:

To study the chemical components and the antifungal activity of extraction from conidia of Trichoderma viride LTR-2. The extraction were obtained by distilling with Methylene dichloride from conidia of Trichoderma viride LTR-2 cultured on wheat bran solid matrix. Antifungal activity were determined by mycelium growth method. The chemical components of the extraction were analysed by GC-MS, the relative components in the extraction were determined by area normalization. The extraction not only have broad-spectrum control, showed antibiosis against eleven different plant fungal pathogens in PDA dish, such as Rhizoctonia solani, Alternaria brassica, Verticillium dahliae, Macrophoma kawatsukai, Fusarium moniliforme, Botrytis cinerea, Rhizoctonia cerealis, Fusarium oxysporum f.sp.vasinfectum, Bipolaris sorokinana, Fusarium graminearum, Alternaria.mali, but also have high inhibitory effect, and had 89.3% suppressive rate to Rhizoctonia cerealis. About sixty components were separated and identified by GC-MS, majority components were Hydrocarbon, the number of the Hydrocarbon were fourty-three kinds. Ergosterol was the major chemical components of the extract, and has 41.90% content. Other components comprised: Ketone, Organic acid, Alcohol, Ene, et al. Conclusion: The extraction from conidia of Trichoderma viride LTR-2 have antifungal activity. The extration comprised 2H-Pyran-2-one, 5, 6-dihydro-6-pentyl, it has 2.35% content. reference others literature, 2H-Pyran-2-one, 5, 6-dihydro-6-pentyl may be the suppressive component of the extration.