肠溶衣聚合物固定化纤维素酶性质的研究

Studies on the Properties of Enteric Coating Polymer Immobilized Cellulase

摘要:

选用国内固定化酶方面研究较少的EudragitL-100为载体，采用物理吸附法，制备出具有可溶-不可溶性质的固定化纤维素酶。固定化酶的溶解度变化的条件是:pH≥5.0时，呈可溶性；pH≤4.0时，呈不可溶性。固定化酶的稳定性较好，重复利用4次后，酶活力保留值在65％以上。比较了固定化酶和游离酶的最适反应条件和动力学常数的大小。以2％的滤纸为底物(15FPU/g底物)，固定化酶水解反应的效果比游离酶好。该研究结果在提高纤维素原料酶水解工艺的经济性方面具有重要意义。

Abstract:

Cellulase was immobilized on the EudragitL-100 by adsorption. The soluble-insoluble immobilized cellulase was gotten. The operational condition of immobilized enzyme is: pH≥5.0, soluble; pH≤4.0, insoluble. The immobilized enzyme was stable. When the immobilized enzyme was reused four times, more than 65％ of the enzyme activity was found to be retained on binding to Eudragit. Kinetic properties and optimal conditions for activity of the immobilized enzyme were compared with that of the native enzyme. This research results were meaningful in the conversion and utilization of renewable biomass.