两株椰心叶甲分离物的鉴定及其系统发育分析

The Identification of Two Isolates from Cadaver of Brontispa longissima(Gestro) and Their Phylogeny

摘要:

采用形态学方法对2株从自然罹病死亡的椰心叶甲虫尸上分离到的致病菌株Dz01和Ma4进行了鉴定，发现2个菌株在菌丝、瓶梗和分生孢子等形态特征上与金龟子绿僵菌小孢变种基本一致，可将2个菌株鉴定为金龟子绿僵菌小孢变种。基于Dz01和Ma4菌株和其它31个代表绿僵菌主要种或变种菌株rDNA上ITS1-5.8S-ITS2区序列构建的最大简约树显示，Dz01和Ma4菌株均聚在金龟子绿僵菌小孢变种所构成的分支中，这为2个菌株形态学鉴定结果提供了分子依据。

Abstract:

The strains Dz01 and Ma4 were isolated from cadavers of Brontispa longissima(Gestro), and were confirmed to be pathogenicity to Brontispa longissima(Gestro). After microscopical observation of the morphological characters of mycelium, phialide and conidia from two isolates, they were found to be identical to Metarhizium anisopliae var anisopliae, so they were identificated as M.anisopliae var anisopliae. The Maximum Parsimony tree constructed based on the sequences of ITS1-5.8S-ITS2 regions in ribosomal DNA from two isolates and 31 other isolates which represent different species or varity species of genus Metarhizium obtained from GenBank database showed that two isolates clustered together in the clade which was composed of the isolates classified as Metarhizium anisopliae var anisopliae. This provided the molecular data for the result of morphological identification of Dz01 and Ma4 isolates.