多重RT-PCR用于临床检测三种胃肠炎病毒的研究

Studies on Simultaneous Detection of Three Gastroenteritis Virus in Clinical Samples by Muptiplex RT-PCR

摘要:

轮状病毒、诺瓦克病毒和星状病毒是引起病毒性胃肠炎的主要病原因子。研究采用JV12/JV13、P1/P2和Mon340/Mon348 三对引物，建立了同时检测这3种病毒的多重RT-PCR技术，并应用于128份临床粪便样本的检测，检出轮状病毒62份(48.44％)，诺瓦克病毒8份(6.25％)，星状病毒11份(8.59％)。在灵敏度试验中，轮状病毒的检测灵敏度为5pg/mL、诺瓦克病毒和星状病毒的检测灵敏度均为50pg/mL。该研究所建立3种常见胃肠炎病毒的多重RT-PCR方法具有特异性强、灵敏度高的特点，可用于临床病原诊断和溯源。

Abstract:

Rotavirus, norwalk virus and astrovirus are the major causes of viral gastroenteritis. Primers JV12/JV13, P1/P2 and Mon340/Mon348 were used to develop a multiplex RT-PCR system to simultaneously detect norwalk virus, rotavirus and astrovirus in clinical fecal samples. In a total of 128 samples, 62 was rotavirus, 8 was norwalk virus and 11 was astrovirus. The detection limits was 5pg/mL for rotavirus, 50pg/mL for norwalk virus and astrovirus respectively. The multiplex RT-PCR system can be used to detect norwalk virus, rotavirus and astrovirus for routine monitoring and risk assessment in diseases outbreak with high specificity and sensitivity.