The aseptic status of microalgaes is regarded as the further research base of physiological and genetic characteristics of microalgaes.The research development of aseptic purification technique of microalgaes in recent years was introduced,including pouring and streaking plate technique,centrifugal washing technique,dilution and filting method,radiation technique,capillary isolation technique,antibiotics technique,chemical disinfectant technique,and the technique based on the utilization of the other properties of microalgaes.