一种快速筛选产琥珀酸厌氧菌的方法

A rapid Screening Procedure for Identification of Succinate Producing Anaerobic Strains

摘要:

为筛选得到高产琥珀酸的厌氧菌株，建立了一种快速半定量分析发酵液中琥珀酸的方法。首先通过高效液相色谱法（HPLC）分析出产琥珀酸厌氧菌发酵液中主要含有琥珀酸，乳酸和乙酸，然后将发酵液经过纸层析展开后，发现琥珀酸可以和副产物乳酸，乙酸完全分开，最后用半定量的方法求出琥珀酸的含量。结果表明纸层析法简单经济，可以作为产琥珀酸厌氧菌的初筛方法。

Abstract:

In order to obtain an efficient screening procedure for identification of succinate producing anaerobic strains，a semi-quantitative paper chromatography method was developed. Lactic acid and acetic acid were identified as the main byproducts in the process of succinate production by the high performance liquid chromatography (HPLC).Succinic acid was completely separated from the byproducts of lactic acid and acetic acid in the same broth developed by paper chromatography.The content of succinic acid was calculated by a semi-quantitative method.The results showed that paper chromatography was a simple and cost effective method that could be utilized to screen anaerobic strains producing succinic acid.