溶氧对L-苏氨酸发酵的影响

The Effect of Dissolved Oxygen on L-Threonine Fermentation

摘要:

探索溶氧对L-苏氨酸发酵过程的影响及其控制方法。通过摇瓶装液量试验、不同溶氧控制方式考察发酵过程中溶氧对L-苏氨酸合成的影响。采用补料分批发酵工艺发酵L-苏氨酸，利用氨基酸分析仪测定发酵液中L-苏氨酸的产量，通过10L罐补料分批发酵36h，产酸可达118.9g/L，糖酸转化率为47.6%。可以得出溶氧对L-苏氨酸生物合成有重要影响，并建立了最佳溶氧控制条件。

Abstract:

To explore the effects of dissolved oxygen on the fermentation of L-threonine and the method for controlling dissolved oxygen.The influence of dissolved oxygen on the synthesis of L-threonine during fermentation was studied under various volumes，various oxygen supply manners.L-threonine was produced by fed-batch fermentation styles，L-threonine fermentation concentration in fermention broth was determined with the methods of amino acid analysis system.The productivity of L-threonine was 118.9g/L in 10-liter fermentor in 36 h，the conversion rate of glucose is 47.6%.Dissolved oxygen influenced the synthesis of L-threonine significantly.A fermentation process for controlling dissolved oxygen was developed.