ARDRA联合RAPD对不动杆菌基因型鉴定的研究

Combination of ARDRA and RAPD Genotyping Technique in Acinetobacter spp.Genomic Species Identification

摘要:

收集多重耐药的不动杆菌10株，以标准参照株作对照，采用扩增核糖体DNA限制性酶切（ARDRA）DNA指纹技术联合随机扩增多态性DNA（RAPD）技术对其基因亚型进行分析；以非加权组间平均法（UPGMA）进行聚类分析。该法可以有效地鉴定不动杆菌基因亚型；并从10株不动杆菌中鉴定出1株琼氏不动杆菌及9株鲍曼不动杆菌。ARDRA联合RAPD基因指纹分型技术有良好的互补性，可准确鉴定不动杆菌基因型。

Abstract:

A total of 10 nonrepetitive multidrug-resistant Acinetobacter strains were collected.With the reference of A.calcoaceticus (ATCC23055)，A.baumannii (ATCC19606) A.lwoffii (ATCC17986) and A.junii (NCTC5866)，DNA fingerprint techniques ARDRA (Amplified ribosomal DNA restriction Analysis) and RAPD (Random amplified polymorphism DNA) were carried out to identify the genomic species of Acinetobacter spp.The distances between them were calculated by unweighted pair group method with arithmetic (UPGMA).Genotypes of Acinetobacter spp.can be effectively classified and an A.junii together with nine A.baumannii isolates was genomically identified.The combination of ARDRA and RAPD DNA-fingerprint technique showed high complementarity，it could be a useful tool in Acinetobacter genomic species identification.