丙酸絮凝发酵新工艺研究

Study on A New Method of Propionic Acid Flocculated Fermentation

摘要:

报道了丙酸发酵的一种新工艺：絮凝发酵工艺。采用谢氏丙酸杆菌（Propionibacterium shermanii）W125在批次发酵产酸达到29g/L的基础上，选择氢氧化钙作为中和剂兼絮凝剂，建立了絮凝半连续发酵工艺，连续运行250h，产酸量达到了35.4g/L，产酸率提高了22%，糖酸转化率达到了51.56%，体积效率达到了0.37g/（L/h）。

关键词:丙酸发酵，絮凝发酵，半连续发酵

Abstract:

This paper reports the flocculated fermentation as a new method of propionic acid production.Propionibacterium shermanii W125 were used，29.0g/L propionic acid was accumulated in batch fermentation.In the subsequently established fed batch semicontinous fermentation，35.4g/L propionic acid was accumulated，raised to 122% and the conversion rate of glucose to propionic acid reached 51.56%，the volumetric productivity reached 0.37g/（L/h）during the 250 hours running cycle.

Key words:Propionic acid fermentation，Flocculated fermentation，Semicontinuous fermentation