泡桐丛枝植原体16S rDNA和延伸因子基因序列分析

Homologic analysis of 16S rDNA and Elongation Factor Gene of Paulownia Witches'Broom Phytoplasma

摘要:

对采自陕西、山西、甘肃、河南、河北、山东各省的泡桐丛枝病材料，利用巢式扩增，均得到16S rDNA基因片段约1.2kb；扩增得到植原体延伸因子(EF-Tu) tuf基因，长度约为850bp.。通过将16S rDNA基因片段和延伸因子(EF-Tu) tuf基因序列与已知植原体16S r各组成员进行同源性比较，确定我国大陆泡桐主栽区陕西、山西、甘肃、河南、河北、山东各省与已经报道的台湾省泡桐丛枝植原体基本一致，均为同一个种，没有株系的分化，全部归属于植原体16Sr I-D组，从而确定了其分类地位。

Abstract:

A 1.2kb of 16S rDNA length frgement is obtained by nest polymerase chain reaction from paulownia witches phytoplasma samples which were collected from Shaanxi，Shanxi，Gansu，Henan，Hebei，Shandong Province，and also a 850bp DNA fragement of tuf (EF-Tu) gene was amplified from above samples using primers (fTufu/rTufu). Homologic analysis shows that it is the same of 16S rDNA nucleotide sequences of PaWB-Shaanxi，PaWB-Taiwan and that of PaWB from Shanxi，Gansu，Henan，Hebei，Shandong Province.All species of PaWB phytoplasma，from different regions，are believed to be a 16Sr I-D group of phytoplasma.Therefore，we can confirm classified position of PaWB phytoplasma from China.