无机磷分解菌BL-11的鉴定及其解磷能力研究

Studies on the Capacity of Phosphate Dissolving and the Identification of Strain BL-11

摘要:

研究了解磷菌株BL-11的菌体形态、生理生化特性，结合该菌的16S rDNA序列分析结果，将菌株BL-11鉴定为侧孢短芽孢杆菌。菌株BL-11在以Ca₃(PO₄)₂为唯一磷源的培养液中的可溶性磷得率为10.91%；在以砂子为唯一磷源的培养液中，可溶性磷得率为1.56%。分解Ca₃(PO₄)₂的最佳条件为30℃，180r/min，pH 7-8；最佳培养基配方为蔗糖20g/L，(NH₄)₂HCO₃ 0.3g/L，MgSO₄·7H₂O 0.5g/L，NaCl 0.3g/L，KCl 0.5g/L，FeSO₄ 0.03g/L，MnSO₄·H₂O 0.03g/L。

Abstract:

The morphology，cultural characteristics，physiological and biochemical properties of phosphate solubilizing strain BL-11 were studied.Combine with its 16S rDNA sequence analysis result strain BL-11 was identified as Brevibacillus laterosporus.Results showed that the phosphate solubilizing efficiency of strain BL-11was up to 10.91% when with Ca₃(PO₃)₂ as the sole phosphorus source in the medium，while the efficiency of that was up to 1.56% when with sands as the sole phosphorus source.An optimal media of BL-11 strain for solubilizing phosphate was obtained by orthogonal test.It was composed of sugar 20g/L，(NH₄)₂HCO₃ 0.3g/L，MgSO₄.7H₂O 0.5g/L，NaCl 0.3g/L，KCl 0.5g/L，FeSO₄ 0.03g/L，MnSO₄.H₂O 0.03g/L. While other conditions were consisted of initial pH7.0-8.0，180 r/min，and 30℃.