The phenol degradation in three-phase fluidized-bed was investigated by using immobilized Ralstonia metallidurans CH34 cells with the aim to improve the efficiency of phenol degradation.The results indicated that the abilities to degrade phenol and resistant to heavy metals of R.metallidurans CH34 have been greatly improved by immobilized cells in three-phase fluidized-bed.The immobilized cells were stable after used for 3~4 batches repeatedly.The present results provided the reliable basis for the application of R.metallidurans CH34 in bio-treatment of phenol-containing industrial waster water.