产氢细菌Enterbacter sakazakii HP的分离及产氢特性

Isolation and Characterization of a H2-producing Strain Enterbacter sakazakii HP

摘要:

在自然环境中分离到一株具有高产氢活性的微生物菌株，经细菌鉴定仪及16S rRNA基因序列分析，鉴定该菌株为Enterbacter sakazakii HP。分析了起始pH值、反应温度、碳源、起始糖浓度、起始氧浓度及菌体密度等因素对菌株产氢活性的影响。研究表明，该菌株发酵产氢较适合的条件为：以葡萄糖为产氢底物，起始pH值8.0，菌体密度OD₆₀₀＝0.7，反应温度35℃，糖浓度为0.1mol/L，氧浓度为0％的条件下，此时产氢菌株的最高产氢活性为5.34μmol H₂/h·mg dw，氢的得率为1.94 mol H₂/mol 葡萄糖。

Abstract:

A H₂-producing bacterial strain was newly isolated and identified as Enterbacte sakazakii HP by 16S rDNA sequence analysis and detection by BBLCRYSTAL AUTOREADER.Various factors，including substrates and its concentration，initial pH，temperature and oxygen，on the hydrogen production of E.sakazakii HP have been studied extensively.Among several sugars，glucose was the favorite substrate for hydrogen production.The optimum condition for hydrogen production by Enterbacte sakazakii HP was achieved as：initial pH8.0，cell density OD₆₀₀=0.7，temperature 35℃，glucose concentration 0.1 mol/L，oxygen concentration 0%.Under batch fermentative hydrogen production conditions，the maximal hydrogen production activity and hydrogen yield were obtained as 5.34μmol H₂/h·mg dw and 1.94mol H₂/mol glucose，respectively.The research results suggest that Enterbacter sakazakii HP is an ideal candidater for biological hydrogen production.