利用根霉和酿酒酵母转化生玉米粉为高浓度酒精

Production of High Ethanol Concentration from Raw Corn Flour Using Rhizopus sp.and Saccharomyces cerevisiae

摘要:

利用从自然界中新分离得到的生淀粉酶产生菌-根霉W-08，并首次采用固态和液态相结合的培养方法，使生淀粉糖化酶活量达到72 IU/mL，然后以生玉米粉为底物，利用根酶W-09和酿酒酵母Z-06采用同步糖化与发酵工艺，30℃，48h，发酵醪酒精浓度达到21%（V/V），转化率为理论转化率的94.5%。

Abstract:

A novel raw-starch-digesting glucoamylase producer，Rhizopus sp.W-08，was used in a novel fermentation system of solid-state followed by submerged，and high enzyme activity of 72 IU/mL was obtained.In the following simultaneous saccharification and fermentation process，Saccharomyces cerevisiae Z-06 directly converted raw corn flour to ethanol with the concentration of 21 % (V/V) at 30℃ after 48h.The conversion efficiency of raw corn flour to ethanol was 94.5 % of the theoretical ethanol yield.