浓香型白酒窖泥中兼性厌氧细菌的分离鉴定

Isolation and Identification of Facultative Anaerobes in the Pit Mud of Chinese Luzhou-flavor Liquor

摘要:

为探讨浓香型白酒窖泥中微生物区系的构成，采用传统微生物分类鉴定法，对泸州老窖不同窖龄窖底和窖壁泥样的可培养兼性厌氧细菌进行了分类计数，并初步鉴定到属。平板涂布法计数得出，老窖底样的菌落总数最多，达3.98×10³cfu/g泥，新窖壁样菌落数最少，只有0.24×10³cfu/g泥；经生理生化鉴定，参照伯杰氏细菌鉴定手册等将划线单离后的菌株归为8个属，其大部分属于芽孢杆菌属 (Bacillus) 和芽孢乳杆菌属 (Sporolactobacillus)，也存在假单胞菌属 (Pseudomonas)、梭菌属 (Clostridium)等。

Abstract:

In order to know the composition of bacterial community in the pit mud of Luzhou-flavor liquor，and to study further the relationship between microbial diversity and liquor characteristics，traditional microbial classification and identification methods were carried out to analyze the culturable facultative anaerobes in the mud samples from both bottoms and walls of the pits of different ages.The bacterial strains were identified to genus according to their morphological，physiological and biochemical characteristics.Counted by using dilution-plate method，the total amount of bacterial colonies in the bottom of older pit was found to be the highest，reaching to 3.98 10³cfu/g mud.While the least was found in the wall of younger pit，only 0.24 10³cfu/g mud.So the older the pit mud is，the bigger the bacterial community is.The result of identification showed that most of the strains isolated from pit mud belong to the genera Bacillus and Sporolactobacillus，those belong to genera Mycobacterium，Pseudomonas，Microbacterium，Corynebacterium，Flavobacterium and Clostridium were also existent.