L-组氨酸产生菌的选育及其特性的初步研究

Study on the Breeding of Histidine Producing Mutant and Its Properties

摘要:

以谷氨酸棒杆菌（Corynebacterium glutamicum）HZ4221（TRA^RDCP^RAMTRhistidase^-）为出发菌株，利用亚硝基胍（NTG）诱变选育得到莽草酸缺陷型的渗漏突变株CLW0560，在以葡萄糖为碳源、硫酸铵为氮源的培养基中直接发酵72h，积累L-组氨酸可达5.31g/L。与亲株相比，L-组氨酸产量提高了32.1%，转酮酶比活力下降84.3%。研究了该菌株利用培养基中碳源情况及菌株遗传稳定性，而且考察了金属离子对CLW0560的影响。

Abstract:

A L-histidine producing mutant was derived from Corynebacterium glutamicum HZ4221（TRA^RDCP^RAMTRhistidase^-）by means of mutagenesis with N-methy-N′-nitro- N-nitrosoguanidine（NTG）.Contrast to original strain，the amount of histidine accumulation reached to a level of 5.31g/L in a medium containing 80g/Lglucose and 30g/L ammonium sulfate after cultured for 72 hours; the transketolase activity reduced to a degree of 15.7%.The utilization of the carbon sources，genetic stability，effect of metal ions were also been investigated in this paper.