广西柑橘溃疡病菌菌系分化研究

Studies on Differentiation of Strains of Xanthomonas axonopodis pv.citri in Guangxi

摘要:

从广西北海、桂林地区11个柑橘品种溃疡病材料上分离得到13株致病菌，进行菌系分化研究表明：在所测试的35项生理特征中有25项(柠檬酸盐、丙二酸盐、36℃生长、耐盐性(1~3%)、吐温20、吐温80、水杨素、卫茅醇、葡萄糖、乳糖、纤维二糖、苯丙氨酸、棉子糖、松三糖、侧金盏花醇、菊糖、核糖、甘露醇、阿拉伯糖、海藻糖、蜜二糖、山梨糖、七叶苷、酒石酸盐、明胶)完全相同，10项(醋酸盐、果糖、枸橼酸盐、鼠李糖、木糖、蔗糖、麦芽糖、半乳糖、淀粉、甘露糖)存在差异；16项生化特征测试中有12项(细胞色素C氧化酶、过氧化氢酶、甲基红试验、乙酰甲基甲醇试验、水解七叶苷、果聚糖产生、硝酸盐还原、脲酶、苯丙氨酸脱氨酶、精氨酸双水解酶、卵磷脂酶、明胶水解)完全相同，4项(水解淀粉、吲哚产生、硫化氢产生、石蕊牛奶)存在差异；3种噬菌体鉴定试验能够区分出13个菌株菌系分化现象的存在，且分为6种敏感型，而菌株细胞脂肪酸成分分析则不能区分菌系分化情况。

关键词:柑橘溃疡病，生理生化测试，噬菌体敏感性，脂肪酸分析，菌系分化

Abstract:

Thirteen strains isolated from diseased materials of 11 citrus varieties collected from Beihai and Guilin districts were studied.Among 35 physiological characteristics assayed in 13 strains，25 items including citrate，malonate，growth at 36℃，NaCl tolerance，Tween 20，Tween 80，salicin，dulcitol，glucose，lactose，cellubiose，phenylalanine，raffinose，melezitose，adonitol，synanthrin，ribose，mannitol，arabinose，trehalose，melibiose，sorbitol，aesculin，tartrate，gelatin were identical and 10 items including acetate，D-fructose，valerate，rhamnose，xylose，sucrose，maltose，galactose，starch，mannose were different; 12 items including cytochrome C oxidase，catalase，MR，VP，hydrolysis of aesculin，production of levan，reduction of nitrate，production of urease，phenylalanine，arginine dihydrolase，lecithinase，hydrolysis of gelatin identical and 4 items including hydrolysis of starch，production of indole，production of H2S，litmus milk different among 16 biochemical ones in strains.The 13 strains could be differentiated by their sensitivities to 3 phages and be divided into 6 types，while no difference could be detected among the strains by the fatty acid profiles.

Key words:Citrus bacterial canker，Physiological and biochemical test，Phage sensitivity，Fatty acid profile，Strain differentiation