Zwittermicin A的分离纯化及其稳定性的初步研究

Study on Separation and Purification of Zwittermicin A and its Stability

摘要:

通过离子交换法，用D113树脂对苏云金芽孢杆菌库斯塔克亚种D1-23发酵液进行初步提纯，经HPLC-MS纯化和制备，得到2.89mg Zwittermicin A纯品。离子交换第1次冲洗液NH₄H₂PO₄的最适浓度为5mmol/L；第2次冲洗液CH₃COONH₄的最适浓度为30mmol/L，梯度冲洗pH值为8.0～9.5； Zwittermicin A的总收得率达93%。Zwittermicin A的热稳定性和pH值稳定性实验表明，Zwittermicin A在100℃下的半衰期为48.22min；在pH值3.0～12.0范围内pH值越低，Zwittermicin A的稳定性越强。

Abstract:

Zwittermicin A was purified by ion exchange resin and HPLC from supernatants of Bacillus thuringiensis.subsp.kurstaki strain D1-23 cultivation.2.89mg pure Zwittermicin A was acquired ，proved by HPLC-MS.Results show that the optimized wash concentration of NH₄H₂PO₄ is 5mmol/L at first step.Next step CH₃COONH₄ concentration is 30 mmol/L，the gradient pH is 8.0～9.5.Totally 93% Zwittermicin A can be reserved with ion exchange resin.The temperature and pH stability experiments show the half life of Zwittermicin A is 48.22 minutes in 100℃，and it is more stable in lower pH in pH 2.0～12.0.