SARS冠状病毒Nsp14基因的克隆与表达

Cloning and Expression of Nsp14 Gene of SARS Coronavirus

摘要:

对SARS冠状病毒的非结构蛋白Nsp14的基因全长进行了克隆表达。根据公布的SARS冠状病毒的非结构蛋白Nsp14的基因序列设计引物，用PCR的方法把该基因从SARS冠状病毒的cDNA片段中扩增出来，经限制性内切酶BamHI与XhoI双酶切后插入到原核表达载体pET30a(+)中，建成重组载体pET30a-Exo。重组载体转化大肠杆菌并进行诱导表达，通过原核表达的方法得到了该蛋白。这为该蛋白的进一步研究奠定了基础。

Abstract:

The Nsp14 gene of SARS coronavirus was cloned and expressed.On the basis of the reported amino acid sequence of the Nsp14 gene of SARS coronavirus，the primers were designed，the gene was cloned from the cDNA segment of SARS coronavirus by PCR，The target fragment was digested with BamHI and XhoI. Nsp14 gene was inserted into the prokaryotic expression vector pET30a to construct recombinant vector pET30a-Exo.Then，the competent E.coli cells were transformed by the recombinant vector and induced by IPTG.The Nsp14 were obtained in the form of fusion protein.It is a promising candidate for further studies.