异育银鲫肠道蛭弧菌的分离及其生物学特性的研究

Carassius auratus gibelio; Bdellovibrio bacteria; Isolate; Biological characteristics

摘要:

以一株具有致病力的温和气单胞菌作为筛选宿主菌,从异育银鲫肠道中分离到一株蛭弧菌,暂命名为BDF-H16。通过光学显微镜、相差显微镜、电子显微镜对蛭弧菌BDF-H16进行形态观察,并研究了其部分生物学特性。结果表明:蛭弧菌BDF-H16为革兰氏阴性菌,杆形或弧杆形,端生一根鞭毛,菌体大小多为0．2μm～0．5μm×0．8μm～1．2μm;蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用;以大肠杆菌为宿主菌,其最佳生长条件为宿主菌浓度6．75×10⁹cfu/mL、pH7．0～7．5、温度28℃;在NaCl含量0．85%～5．00%的培养基中能够生长;恩诺沙星、诺氟沙星对其有抑制作用。

Abstract:

Bdellovibrio bacteria BDF-H16 was isolated from the gut of Carassius auratus gibelio with Aeromonas sobria.Its shape was ob- served by light microscopy,phase-contrast microscopy,electron microscopy and some of its biological characteristics were also studied.It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.Its size was mostly 0.2μm～0.5μm×0.8μm～1.2μm.It had a wide prey area and could lyse all tested gram-negative bacteria and some gram-positive bacteria. The best lysis conditions to Escherichia coli were 6.75×10⁹cfu/mL of prey bacteria concentration、pH7.0~7.5、28℃. It could grow in the solid culture added 0.85%~5.00% NaCl and was inhibited by enrofloxacin and norfloxacin.