常压室温等离子体结合高通量筛选技术在微生物育种方面的研究进展

Research progress in atmospheric and room-temperature plasma combined with high-throughput screening in microbial breeding

摘要:

常压室温等离子体(atmospheric and room temperature plasma, ARTP)是一种新型诱变技术，具有诱变速度快、诱变条件温和、环境友好等特点。由于ARTP诱变的突变体具有高突变频率和良好的遗传稳定性，因此在微生物育种领域应用广泛。本文综述了近年来ARTP诱变技术在微生物育种中取得的进展、诱变后突变菌株的筛选策略和ARTP诱导微生物发生突变的机制，并对ARTP目前在微生物诱变育种中存在的问题进行讨论。

关键词:ARTP;微生物育种;高通量筛选;突变机制

Abstract:

Atmospheric and room temperature plasma (ARTP), a new approach of mutagenesis, is characterized by fast mutagenesis speed, mild mutagenesis conditions, and environmental friendliness. The ARTP-induced mutants have high mutagenesis frequency and good genetic stability. Therefore, ARTP is widely used in microbial breeding. This paper reviews the progress in ARTP in microbial breeding in recent years, the screening strategy of mutants after mutagenesis, and the mechanism of ARTP-induced mutagenesis in microorganisms. Finally, we discuss the existing problems of ARTP in microbial breeding.

Key words:atmospheric and room temperature plasma (ARTP);microbial breeding;high-throughput screening;mutation mechanism