科微学术

抗菌活性海洋真菌HN4-13的鉴定及其发酵优化
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连云港市科技攻关项目(No. CG1131); 国家暨江苏省大学生实验创新项目(No. 201211641008)


Identification and fermentation optimization of marine fungus HN4-13 with antibacterial activity
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    摘要:

    【目的】鉴定一株来源于连云港近岸海域沉积物并具有分泌抗菌活性代谢产物的真菌菌株HN4-13, 优化其合成抗菌活性物质的发酵条件。【方法】通过形态学观察及ITS 序列分析方法对菌株HN4-13进行鉴定; 通过基础发酵培养基筛选、碳氮源及无机盐的单因素试验, 继而采用正交试验设计优化其合成抗菌活性物质的发酵条件。【结果】菌株HN4-13被鉴定为黄柄曲霉(Aspergillus flavipes), 其分泌抗菌活性产物的发酵条件为: 4%蔗糖、0.5%蛋白胨、0.1% KCl、0.06% NaH2PO4、28 °C、1%接种量、160 r/min培养9?d。【结论】实验结果为进一步分离纯化菌株HN4-13所产抗菌活性代谢产物提供了基础。

    Abstract:

    [Objective] To identify marine fungus HN4-13 isolated from Lianyungang coastal sea sediments with antibacterial activity and to optimize the fermentation conditions of synthesizing antibacterial active substances. [Methods] Strain HN4-13 was identified based on its morphological characters and internal transcribed spacer (ITS) sequence; The optimization of fermentation conditions for antibacterial production by strain HN4-13 were investigated by the one-factor-at-a-time method and the orthogonal design method. [Results] Strain HN4-13 was identified as Aspergillus flavipes, and the optimal fermentation conditions can be recognized as follows: 4% sucrose, 0.5% peptone, 0.1% KCl, 0.06% NaH2PO4, 1% inoculation concentration, 28 °C, 160 r/min for 9 days. [Conclusion] The results provide the clues for further separation and purification of the antibacterial active metabolites derived from strain HN4-13.

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郭雷,朱文成,刘玮炜,丁国伟,张越乾,张守禄,邱月,谢宇.抗菌活性海洋真菌HN4-13的鉴定及其发酵优化[J].微生物学通报,2013,40(6):951-958

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  • 在线发布日期: 2013-06-04
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